dunn chamber Search Results


direct-viewing dunn chambers  (Weber Scientific International Ltd)
90
Weber Scientific International Ltd direct-viewing dunn chambers
Direct Viewing Dunn Chambers, supplied by Weber Scientific International Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/direct-viewing dunn chambers/product/Weber Scientific International Ltd
Average 90 stars, based on 1 article reviews
direct-viewing dunn chambers - by Bioz Stars, 2026-02
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dunn chemotaxis chamber  (Weber Scientific International Ltd)
90
Weber Scientific International Ltd dunn chemotaxis chamber
Dunn Chemotaxis Chamber, supplied by Weber Scientific International Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dunn chemotaxis chamber/product/Weber Scientific International Ltd
Average 90 stars, based on 1 article reviews
dunn chemotaxis chamber - by Bioz Stars, 2026-02
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dunn chamber  (Hawksley Sons)
90
Hawksley Sons dunn chamber
Expression of WASP L46P or WASP A47D does not rescue the chemotaxis defect of Jurkat WASP-KD T cells. A) Vector plots of migration paths of 20 randomly chosen cells from Jurkat WASP-KD T cells expressing RFP, WASP R -RFP, WASP R L46P -RFP, WASP R A47D -RFP exposed to a gradient of SDF-1α in the <t>Dunn</t> <t>chamber.</t> Time-lapse images were captured for 3 hours at 2.0 min intervals. The starting point of each cell is at the intersection of the X and Y axes. The source of SDF-1α was at the top. B) Migration velocity of each cell type as in panel A, mean of velocities of 60 randomly chosen cells (20 cells each from 3 sets of experiments) * P < 0.05 compared to Jurkat WASP-KD T cells expressing WASP R -RFP. C) Circular histograms showing percentage of cells at their final positions in each of the sectors (20°). The source of SDF-1α was at the top. D) In transwell migration assay, cells were loaded in upper well and allowed to migrate towards SDF-1α (100 ng/ml) containing media in the lower well for 3.0 hours. Cells migrated to the lower well were counted and the percentage of cells migrated to the lower well was calculated. Data represent the mean of three independent experiments. * P < 0.05, ** P < 0.01 .
Dunn Chamber, supplied by Hawksley Sons, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dunn chamber/product/Hawksley Sons
Average 90 stars, based on 1 article reviews
dunn chamber - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
dunn chamber  (Weber Scientific International Ltd)
90
Weber Scientific International Ltd dunn chamber
Expression of WASP L46P or WASP A47D does not rescue the chemotaxis defect of Jurkat WASP-KD T cells. A) Vector plots of migration paths of 20 randomly chosen cells from Jurkat WASP-KD T cells expressing RFP, WASP R -RFP, WASP R L46P -RFP, WASP R A47D -RFP exposed to a gradient of SDF-1α in the <t>Dunn</t> <t>chamber.</t> Time-lapse images were captured for 3 hours at 2.0 min intervals. The starting point of each cell is at the intersection of the X and Y axes. The source of SDF-1α was at the top. B) Migration velocity of each cell type as in panel A, mean of velocities of 60 randomly chosen cells (20 cells each from 3 sets of experiments) * P < 0.05 compared to Jurkat WASP-KD T cells expressing WASP R -RFP. C) Circular histograms showing percentage of cells at their final positions in each of the sectors (20°). The source of SDF-1α was at the top. D) In transwell migration assay, cells were loaded in upper well and allowed to migrate towards SDF-1α (100 ng/ml) containing media in the lower well for 3.0 hours. Cells migrated to the lower well were counted and the percentage of cells migrated to the lower well was calculated. Data represent the mean of three independent experiments. * P < 0.05, ** P < 0.01 .
Dunn Chamber, supplied by Weber Scientific International Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dunn chamber/product/Weber Scientific International Ltd
Average 90 stars, based on 1 article reviews
dunn chamber - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
dunn chambers  (Hawksley Sons)
90
Hawksley Sons dunn chambers
Expression of WASP L46P or WASP A47D does not rescue the chemotaxis defect of Jurkat WASP-KD T cells. A) Vector plots of migration paths of 20 randomly chosen cells from Jurkat WASP-KD T cells expressing RFP, WASP R -RFP, WASP R L46P -RFP, WASP R A47D -RFP exposed to a gradient of SDF-1α in the <t>Dunn</t> <t>chamber.</t> Time-lapse images were captured for 3 hours at 2.0 min intervals. The starting point of each cell is at the intersection of the X and Y axes. The source of SDF-1α was at the top. B) Migration velocity of each cell type as in panel A, mean of velocities of 60 randomly chosen cells (20 cells each from 3 sets of experiments) * P < 0.05 compared to Jurkat WASP-KD T cells expressing WASP R -RFP. C) Circular histograms showing percentage of cells at their final positions in each of the sectors (20°). The source of SDF-1α was at the top. D) In transwell migration assay, cells were loaded in upper well and allowed to migrate towards SDF-1α (100 ng/ml) containing media in the lower well for 3.0 hours. Cells migrated to the lower well were counted and the percentage of cells migrated to the lower well was calculated. Data represent the mean of three independent experiments. * P < 0.05, ** P < 0.01 .
Dunn Chambers, supplied by Hawksley Sons, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dunn chambers/product/Hawksley Sons
Average 90 stars, based on 1 article reviews
dunn chambers - by Bioz Stars, 2026-02
90/100 stars
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modified dunn chambers  (Hawksley Sons)
90
Hawksley Sons modified dunn chambers
Expression of WASP L46P or WASP A47D does not rescue the chemotaxis defect of Jurkat WASP-KD T cells. A) Vector plots of migration paths of 20 randomly chosen cells from Jurkat WASP-KD T cells expressing RFP, WASP R -RFP, WASP R L46P -RFP, WASP R A47D -RFP exposed to a gradient of SDF-1α in the <t>Dunn</t> <t>chamber.</t> Time-lapse images were captured for 3 hours at 2.0 min intervals. The starting point of each cell is at the intersection of the X and Y axes. The source of SDF-1α was at the top. B) Migration velocity of each cell type as in panel A, mean of velocities of 60 randomly chosen cells (20 cells each from 3 sets of experiments) * P < 0.05 compared to Jurkat WASP-KD T cells expressing WASP R -RFP. C) Circular histograms showing percentage of cells at their final positions in each of the sectors (20°). The source of SDF-1α was at the top. D) In transwell migration assay, cells were loaded in upper well and allowed to migrate towards SDF-1α (100 ng/ml) containing media in the lower well for 3.0 hours. Cells migrated to the lower well were counted and the percentage of cells migrated to the lower well was calculated. Data represent the mean of three independent experiments. * P < 0.05, ** P < 0.01 .
Modified Dunn Chambers, supplied by Hawksley Sons, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/modified dunn chambers/product/Hawksley Sons
Average 90 stars, based on 1 article reviews
modified dunn chambers - by Bioz Stars, 2026-02
90/100 stars
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dunn cell migration chambers  (Weber Scientific International Ltd)
90
Weber Scientific International Ltd dunn cell migration chambers
Expression of WASP L46P or WASP A47D does not rescue the chemotaxis defect of Jurkat WASP-KD T cells. A) Vector plots of migration paths of 20 randomly chosen cells from Jurkat WASP-KD T cells expressing RFP, WASP R -RFP, WASP R L46P -RFP, WASP R A47D -RFP exposed to a gradient of SDF-1α in the <t>Dunn</t> <t>chamber.</t> Time-lapse images were captured for 3 hours at 2.0 min intervals. The starting point of each cell is at the intersection of the X and Y axes. The source of SDF-1α was at the top. B) Migration velocity of each cell type as in panel A, mean of velocities of 60 randomly chosen cells (20 cells each from 3 sets of experiments) * P < 0.05 compared to Jurkat WASP-KD T cells expressing WASP R -RFP. C) Circular histograms showing percentage of cells at their final positions in each of the sectors (20°). The source of SDF-1α was at the top. D) In transwell migration assay, cells were loaded in upper well and allowed to migrate towards SDF-1α (100 ng/ml) containing media in the lower well for 3.0 hours. Cells migrated to the lower well were counted and the percentage of cells migrated to the lower well was calculated. Data represent the mean of three independent experiments. * P < 0.05, ** P < 0.01 .
Dunn Cell Migration Chambers, supplied by Weber Scientific International Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dunn cell migration chambers/product/Weber Scientific International Ltd
Average 90 stars, based on 1 article reviews
dunn cell migration chambers - by Bioz Stars, 2026-02
90/100 stars
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Image Search Results


Expression of WASP L46P or WASP A47D does not rescue the chemotaxis defect of Jurkat WASP-KD T cells. A) Vector plots of migration paths of 20 randomly chosen cells from Jurkat WASP-KD T cells expressing RFP, WASP R -RFP, WASP R L46P -RFP, WASP R A47D -RFP exposed to a gradient of SDF-1α in the Dunn chamber. Time-lapse images were captured for 3 hours at 2.0 min intervals. The starting point of each cell is at the intersection of the X and Y axes. The source of SDF-1α was at the top. B) Migration velocity of each cell type as in panel A, mean of velocities of 60 randomly chosen cells (20 cells each from 3 sets of experiments) * P < 0.05 compared to Jurkat WASP-KD T cells expressing WASP R -RFP. C) Circular histograms showing percentage of cells at their final positions in each of the sectors (20°). The source of SDF-1α was at the top. D) In transwell migration assay, cells were loaded in upper well and allowed to migrate towards SDF-1α (100 ng/ml) containing media in the lower well for 3.0 hours. Cells migrated to the lower well were counted and the percentage of cells migrated to the lower well was calculated. Data represent the mean of three independent experiments. * P < 0.05, ** P < 0.01 .

Journal: Journal of Biomedical Science

Article Title: X-Linked thrombocytopenia causing mutations in WASP (L46P and A47D) impair T cell chemotaxis

doi: 10.1186/s12929-014-0091-1

Figure Lengend Snippet: Expression of WASP L46P or WASP A47D does not rescue the chemotaxis defect of Jurkat WASP-KD T cells. A) Vector plots of migration paths of 20 randomly chosen cells from Jurkat WASP-KD T cells expressing RFP, WASP R -RFP, WASP R L46P -RFP, WASP R A47D -RFP exposed to a gradient of SDF-1α in the Dunn chamber. Time-lapse images were captured for 3 hours at 2.0 min intervals. The starting point of each cell is at the intersection of the X and Y axes. The source of SDF-1α was at the top. B) Migration velocity of each cell type as in panel A, mean of velocities of 60 randomly chosen cells (20 cells each from 3 sets of experiments) * P < 0.05 compared to Jurkat WASP-KD T cells expressing WASP R -RFP. C) Circular histograms showing percentage of cells at their final positions in each of the sectors (20°). The source of SDF-1α was at the top. D) In transwell migration assay, cells were loaded in upper well and allowed to migrate towards SDF-1α (100 ng/ml) containing media in the lower well for 3.0 hours. Cells migrated to the lower well were counted and the percentage of cells migrated to the lower well was calculated. Data represent the mean of three independent experiments. * P < 0.05, ** P < 0.01 .

Article Snippet: The outer and inner well of Dunn chamber (Hawksley & Sons Ltd, UK) [ ] were filled with complete RPMI media.

Techniques: Expressing, Chemotaxis Assay, Plasmid Preparation, Migration, Transwell Migration Assay

WASP knockdown Jurkat T cells are impaired in chemotaxis towards SDF-1α. A) Jurkat T cells were transduced with retrovirus expressing human WASP specific shRNA and EGFP. The cells were FACS sorted and the efficiency of knock down was determined by western blot using anti-WASP and anti-GAPDH antibody. B) WASP mRNA levels in Jurkat T cells and Jurkat WASP-KD T cells were quantified by real-time PCR. ***P < 0.001 compared to WT Jurkat T cells. C) Vector plots of migration paths of 20 randomly chosen cells from WT Jurkat T cells or Jurkat WASP-KD T cells exposed to a gradient of SDF-1α in the Dunn chamber. The starting point of each cell is at the intersection of the X and Y axes. The source of SDF-1α was at the top. D) Migration velocity of WT and Jurkat WASP-KD T cells shown as the mean of the velocities of 60 randomly chosen cells (20 cells each from 3 sets of experiments) * p < 0.05 . E) Circular histograms showing the percentage of cells at the final positions in each of the sectors (20°). The source of SDF-1α was at the top. F) Transwell migration assay was performed with WT Jurkat T cells and Jurkat WASP-KD T cells. 2 X 10 5 cells were loaded in upper well and allowed to migrate towards SDF-1α (100 ng/ml) containing media in the lower well for 3.0 hrs. Cells migrated to the lower well were counted and plotted as percentages of total cells added to the upper well. Data represent the mean of three independent experiments. **p < 0.01 compared to WT Jurkat T cells.

Journal: Journal of Biomedical Science

Article Title: X-Linked thrombocytopenia causing mutations in WASP (L46P and A47D) impair T cell chemotaxis

doi: 10.1186/s12929-014-0091-1

Figure Lengend Snippet: WASP knockdown Jurkat T cells are impaired in chemotaxis towards SDF-1α. A) Jurkat T cells were transduced with retrovirus expressing human WASP specific shRNA and EGFP. The cells were FACS sorted and the efficiency of knock down was determined by western blot using anti-WASP and anti-GAPDH antibody. B) WASP mRNA levels in Jurkat T cells and Jurkat WASP-KD T cells were quantified by real-time PCR. ***P < 0.001 compared to WT Jurkat T cells. C) Vector plots of migration paths of 20 randomly chosen cells from WT Jurkat T cells or Jurkat WASP-KD T cells exposed to a gradient of SDF-1α in the Dunn chamber. The starting point of each cell is at the intersection of the X and Y axes. The source of SDF-1α was at the top. D) Migration velocity of WT and Jurkat WASP-KD T cells shown as the mean of the velocities of 60 randomly chosen cells (20 cells each from 3 sets of experiments) * p < 0.05 . E) Circular histograms showing the percentage of cells at the final positions in each of the sectors (20°). The source of SDF-1α was at the top. F) Transwell migration assay was performed with WT Jurkat T cells and Jurkat WASP-KD T cells. 2 X 10 5 cells were loaded in upper well and allowed to migrate towards SDF-1α (100 ng/ml) containing media in the lower well for 3.0 hrs. Cells migrated to the lower well were counted and plotted as percentages of total cells added to the upper well. Data represent the mean of three independent experiments. **p < 0.01 compared to WT Jurkat T cells.

Article Snippet: The outer and inner well of Dunn chamber (Hawksley & Sons Ltd, UK) [ ] were filled with complete RPMI media.

Techniques: Knockdown, Chemotaxis Assay, Transduction, Expressing, shRNA, Western Blot, Real-time Polymerase Chain Reaction, Plasmid Preparation, Migration, Transwell Migration Assay